Ribonuclease E, an Intermediate in the Degradation of Ribonuclease by Porcine Elastase
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چکیده
منابع مشابه
Ribonuclease E, an Intermediate in the Degradation of Ribonuclease by Porcine Elastase.
Bovine pancreatic ribonuclease has been studied as the substrate of a number of proteolytic enzymes. In the native state it is completely resistant to trypsin and chymotrypsin (1, 2), but denaturation by chemical (3) or physical (4, 5) means induces susceptibility to these enzymes. Ribonuclease is! however, susceptible to attack by a number of other proteases. Subtilisin has been shown to cause...
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Porcine pancreatic RNase was isolated from the pancreatic secretion of animals with permanent fistulas by a combination of DEAE-cellulose chromatography, adsorption-elution from sulfoethyl-Sephadex, and gel filtration on Sephadex G-100. On the basis of these experiments, a practicable, large scale procedure was developed for the preparation of the enzyme in quantities of 100 to 200 mg. Porcine ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1965
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)97265-0